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McCarthy et al. (2001) tested a taurine assay in pet food after acid hydrolysis (6 M HCl, 110°C, 16 h) and precolumn derivatization with dansyl chloride in a collaborative trial. 3% were achieved, and the procedure was adopted as the official method by AOAC International. Porter et al. (1988), Amiss et al. (1990), Sakai and Nagasawa (1992) and Messing and Sturmann (1993) described the determination of taurine in feline and other blood and plasma by HPLC separation following precolumn derivatization with different reagents.

D. R. (2001) Free amino acids in dark- and white-muscle fish as determinated by o-phthaldialdehyde precolumn derivatization. Journal of Food Science 66, 72–77. 12 Amino Acids in Feeds. In: Official Methods of Analysis, 16th edn. AOAC International, Arlington, Virginia, chapter 4, 4–4H. -C. and Toldra, F. (1991) Deproteinization for HPLC amino acid analysis in fresh pork muscle and dry-cured ham. Journal of Agricultural and Food Chemistry 39, 1792–1795. D. and Martelli, A. (1998) Ion-pair HPLC determination of biogenic amines and precursor amino acids.

The advantage is that it eliminates the need for rotary removal of the halogen prior to hydrolysis and that phenylalanine and histidine can also be determined from the oxidized hydrolysate. This method is capable of determining all amino acids except tryptophan and tyrosine. However, it is important to remember that these hydrolysates now contain sulphuric acid or hydrogen sulphate formed from sulphite which, when concentrated for the complete removal of the hydrochloric acid, can form sulphate esters with serine and threonine which interfere with cysteic acid in the amino acid analyser.

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