Download e-book for iPad: Carotenoids: Nutrition and Health by George Britton, Synnove Liaaen-Jensen, Hanspeter Pfander

By George Britton, Synnove Liaaen-Jensen, Hanspeter Pfander

The Carotenoids book sequence offers distinctive bills of the elemental chemistry of carotenoids and the fundamental tools utilized in carotenoid study, and demanding discussions of the biochemistry, capabilities and functions of those very important compounds.

The ultimate quantity, Volume 5, bargains with the subject that's of maximum common curiosity and public quandary, carotenoids in human healthiness and meals. this can be the period of ‘functional foods’, and deciding upon roles of chemical parts of meals as very important micronutrients. Carotenoids characteristic excessive at the record of those. Volume 5 follows the carotenoid tale from nutrients to organic activities.

Carotenoids, quantity 5 is deliberate as a coordinated, built-in therapy offering updated and demanding study surveys by way of best experts within the box, and incorporating a few heritage fabric to assist make the chapters obtainable to carotenoid researchers who're now not experts at the specific topic.

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Additional resources for Carotenoids: Nutrition and Health

Sample text

HPLC of Carotenoids: General Aspects HPLC of carotenoids has a long and interesting history, beginning in the early 1970s [7]. The first work used ‘home-packed’ columns with stationary phases such as MgO or Ca(OH)2 that had been widely used as TLC and CC adsorbents. These stationary phases were not designed for application in HPLC. They did not have uniform particle size and shape or controlled pore size, and HPLC columns of these adsorbents were not available commercially. Reproducibility between different ‘home-packed’ columns and between batches of the same adsorbent was not good, and factors such as moisture content were difficult to control.

Also each component may be present in the all-E and various Z isomeric forms. Assignment of peaks in such a multicomponent chromatogram is challenging and proper identification is essential. 2. Separation of xanthophylls Good resolution of xanthophylls can be achieved on both NP (silica or bonded nitrile) and RP stationary phases (see Figs. 3 and 4). Much effort has been expended on developing systems for resolving the isomeric pair lutein and zeaxanthin. Most popular for routine use are RP systems, which can give good resolution not only of lutein (133) and zeaxanthin (119) but also of other xanthophylls including antheraxanthin (231), lutein 5,6-epoxide (232), violaxanthin (259) and neoxanthin (234).

When isocratic elution is used, the solvent composition is optimized to give the best resolution of the components of interest. Analysis of Carotenoids in Nutritional Studies 15 Polarity, due to the possession of polar functional groups, is the main determinant of interactions between carotenoids and the polar silanol Si-OH groups of the silica stationary phase. Polar groups or regions of the carotenoid molecule compete with polar solvent modifiers for adsorption sites on the stationary phase. The carotenes, with no polar substituents and overall low polarity, have little affinity for normal phase columns and are eluted almost immediately, so selectivity and resolution are not good.

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